Direct identification of bacteria from BacT/ALERT anaerobic positive blood cultures by MALDI-TOF MS: MALDI Sepsityper kit versus an in-house saponin method for bacterial extraction - Peeref (2024)

Article Biology

Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry

Gabrijela Perse, Ivana Samoscanec, Zrinka Bosnjak, Ana Budimir, Tomislav Kulis, Ivana Marekovic

Summary: This study evaluated an in-house method for identifying bacteria directly from positive blood cultures, compared to the Sepsityper (R) Kit, and assessed the benefit of on-plate formic acid extraction. The results showed that the in-house method had lower identification rates for Gram-positive bacteria at the genus and species level, but the addition of on-plate formic acid extraction significantly improved the identification rate.

Article Microbiology

Identification of micro- organism from positive blood cultures: comparison of three different short culturing methods to the Rapid Sepsityper workflow

Arthur B. Pranada, Miriam Cordovana, Markus Meyer, Hilke Hubert, Mohammad Abdalla, Simone Ambretti, Joerg Steinmann

Summary: This study compared the effectiveness of the Rapid Sepsityper workflow and short subculturing methods in isolating and identifying causative micro-organisms. The results showed that, in most cases, the Rapid Sepsityper workflow outperformed the short subculturing methods and provided faster results.

Article Immunology

Evaluation of the Rapid Sepsityper protocol and specific MBT-Sepsityper module for the identification of bacteremia and fungemia using Bruker Biotyper MALDI-TOF MS

Hsiu-Hsien Lin, Kun-Hao Tseng, Ni Tien, Yu-Tzu Lin, Jiaxin Yu, Po-Ren Hsueh, Der-Yang Cho

Summary: The rapid Sepsityper protocol based on the MALDI Biotyper platform accurately identifies the species of microorganisms in blood culture samples. Gram-negative bacilli have a higher identification rate compared to Gram-positive cocci and yeasts. However, the identification performance of the rapid Sepsityper protocol is poor for polymicrobial samples.

Article Multidisciplinary Sciences

A streamlined method for the fast and cost-effective detection of bacterial pathogens from positive blood cultures for the BacT/ALERT blood culture system using the Vitek MS mass spectrometer

Johannes Forster, Britta Kohlmorgen, Julian Haas, Philipp Weis, Lukas Breunig, Doris Turnwald, Boris Mizaikoff, Christoph Schoen

Summary: This study establishes an inexpensive, fast, and accurate method for the prompt identification of bacterial species directly from blood culture bottles using BacT/Alert(R) and VitekMS(R). The protocol showed higher success rates in identifying gram-negative bacteria and had shorter processing time compared to other similar methods. The estimated costs per sample were also significantly lower.

Article Immunology

Identification of Peptoniphilus harei From Blood Cultures in an Infected Aortic Aneurysm Patient: Case Report and Review Published Literature

Xue Wan, Shuang Wang, Min Wang, Jinhua Liu, Yu Zhang

Summary: This study reports a case of Peptoniphilus harei bacteremia in an abdominal aortic aneurysm patient, highlighting the importance of including anaerobic blood cultures as part of blood culture procedures. Clinical microbiologists should be vigilant that the pathogenic potential of GPAC may be underestimated.

Article Medicine, General & Internal

Multicenter Evaluation of Rapid BACpro(R) II for the Accurate Identification of Microorganisms Directly from Blood Cultures Using MALDI-TOF MS

Marina Oviano, Andre Ingebretsen, Anne K. Steffensen, Antony Croxatto, Guy Prod'hom, Lidia Quiroga, German Bou, Gilbert Greub, David Rodriguez-Temporal, Belen Rodriguez-Sanchez

Summary: The study evaluated a novel commercial method for rapidly identifying microorganisms directly from blood cultures, showing high rates of correct species-level identification, especially for Gram positive bacteria.

Article Infectious Diseases

Evaluation of the mycobacteria MBT kit for identification of nontuberculous mycobacteria by MALDI-TOF Biotyper (Bruker)

Emilie Cardot Martin, Lucie Limousin, Claudine Renaux, Emilie Catherinot, Marc Vasse

Summary: This study compared the performance of the Easy MycoEx protocol and the MycoEx protocol in the identification of mycobacteria. The results showed that the identification rates were similar between the two protocols in MGIT broth, but the reproducibility was superior with the Easy MycoEx protocol on Coletsos agar. The study demonstrated that clinical laboratories can easily implement the Easy MycoEx protocol for mycobacteria identification.

Article Infectious Diseases

Direct Detection of KPC Peak from Positive Blood Cultures Using MALDI-TOF MS: Are We There Yet?

Natalia Kehl Moreira, Camila Morschbacher Wilhelm, Ayme Duarte Echevarria, Fabiana Caroline Zempulski Volpato, Priscila Lamb Wink, Afonso Luis Barth, Juliana Caierao

Summary: Detection of KPC enzyme directly from positive blood cultures using MALDI-TOF MS is feasible and has high sensitivity and specificity. This method can rapidly and accurately assess bacterial resistance mechanisms, which is crucial for selecting appropriate treatment and improving antimicrobial stewardship.

Article Agricultural Engineering

Catalytic hydrogenolysis of lignin in ethanol/isopropanol over an activated carbon supported nickel-copper catalyst

Chongbo Cheng, Pengfei Li, Wenbing Yu, Dekui Shen, Sai Gu

Summary: In this study, catalytic hydrogenolysis of organosolv poplar lignin was conducted using NiCu/C catalysts in ethanol/isopropanol solvent, achieving high yields of phenolic monomers. The introduction of Cu in catalysts promoted the hydrogen donor process of the solvent and facilitated the cleavage of lignin linkages, resulting in decreased molecular weight of bio-oil. The possible lignin dimer type structures were proposed and identified by MALDI-TOF MS, providing a better understanding of NiCu/C catalyzed lignin depolymerization.

Article Microbiology

Rapid Classification of Clostridioides difficile Strains Using MALDI-TOF MS Peak-Based Assay in Comparison with PCR-Ribotyping

Adriana Calderaro, Mirko Buttrini, Monica Martinelli, Benedetta Farina, Tiziano Moro, Sara Montecchini, Maria Cristina Arcangeletti, Carlo Chezzi, Flora De Conto

Summary: This study evaluated a Matrix-Assisted Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS (T-MALDI)) method for rapid classification of circulating C. difficile strains. The results showed that this method has good recognition and validation capabilities, especially for PR1-PR5 ribotyping.

Article Food Science & Technology

Two-dimensional gel analysis to investigate the effect of hydroxyl radical oxidation on freshness indicator protein of Coregonus peled during 4 °C storage

Xue Li, Xiaorong Deng, Xin Guo, Yabo Wei, Yunfeng Zhao, Xiaobing Guo, Xinrong Zhu, Jian Zhang, Ling Hu

Summary: The present study investigates the freshness indicator protein of Coregonus peled and the effects of oxidation on the quality of fish during 4 degrees C storage. A total of 382 differential proteins were identified, and 20 proteins related to oxidation were found to have the potential as biomarkers for protein oxidative damage. Actin was identified as an indicator for fish softening during postmortem storage. The changes in protein structure and biomarkers were influenced by oxidation. Bioinformatics analysis suggested that differentially expressed proteins participated in the metabolic pathway during 4 degrees C storage.

Article Biochemistry & Molecular Biology

Culturomics Approach to Identify Diabetic Foot Infection Bacteria

Michal Zloch, Ewelina Maslak, Wojciech Kupczyk, Marek Jackowski, Pawel Pomastowski, Boguslaw Buszewski

Summary: This study evaluated the use of different culture media in reflecting the microbial compositions of diabetic foot infections in Poland. The results showed significant differences in species coverage and efficiency between the investigated media. Selecting the appropriate media set can significantly improve the accuracy of reflecting the microbial compositions of diabetic foot infections.

Article Chemistry, Medicinal

An Efficient and Quick Analytical Method for the Quantification of an Algal Alkaloid Caulerpin Showed In-Vitro Anticancer Activity against Colorectal Cancer

Nazli Mert-Ozupek, Gizem Calibasi-Kocal, Nur Olgun, Yasemin Basbinar, Levent Cavas, Hulya Ellidokuz

Summary: The study compared caulerpin levels in invasive and non-invasive Caulerpa extracts for the first time, showing higher levels in the invasive species. Furthermore, caulerpin from invasive Caulerpa demonstrated anticancer activities by reducing cell viability, inhibiting invasion-migration, and inducing apoptosis in colorectal cancer cells.

Article Microbiology

Identification of Filamentous Fungi by MALDI-TOF Mass Spectrometry: Evaluation of Three Different Sample Preparation Methods and Validation of an In-House Species Cutoff

Claudia Honsig, Brigitte Selitsch, Marlene Hollenstein, Matthias G. Vossen, Kathrin Spettel, Birgit Willinger

Summary: In this study, the identification of 230 filamentous fungi isolates using MALDI-TOF MS in combination with the Filamentous Fungi Library 3.0 was investigated. The results showed that using the more time-consuming sample preparation methods improved species-level identification rates, and the in-house threshold for species identification was validated for routine use in the laboratory. This suggests that this commercial platform is suitable for routine diagnosis.

Article Agriculture, Dairy & Animal Science

Research Note: Antimicrobial resistance profile of Enterococcus spp. isolated from the eggshell of laying hens submitted to pharmacological treatment

I. P. Lanza, G. R. Silva, L. D. M. Menezes, D. C. S. Assis, H. C. P. Figueiredo, A. M. Q. Lana, L. J. C. Lara, T. C. Figueiredo, M. R. Souza, S. Cancado

Summary: Enterococcus isolated from eggshells shows significant antimicrobial resistance. The use of antimicrobials in feed leads to multidrug resistance in the isolated Enterococcus. This may be due to environmental factors.

Article Dermatology

Infrared spectroscopy as a novel tool to diagnose onychomycosis

Sander De Bruyne, R. Speeckaert, J. Boelens, M-P Hayette, M. Speeckaert, J. Delanghe

Article Infectious Diseases

Multicentre validation of a EUCAST method for the antifungal susceptibility testing of microconidia-forming dermatophytes

Maiken Cavling Arendrup, Karin Meinike Jorgensen, Jesus Guinea, Katrien Lagrou, Erja Chryssanthou, Marie-Pierre Hayette, Francesco Barchiesi, Cornelia Lass-Floerl, Petr Hamal, Eric Dannaoui, Anuradha Chowdhary, Joseph Meletiadis

Article Infectious Diseases

Molecular surveillance of anti-malarial drug resistance in Democratic Republic of Congo: high variability of chloroquinoresistance and lack of amodiaquinoresistance

Doudou Yobi, Nadine K. Kayiba, Dieudonne M. Mvumbi, Raphael Boreux, Pius Z. Kabututu, Hippolyte N. T. Situakibanza, Joris L. Likwela, Patrick De Mol, Emile W. Okitolonda, Niko Speybroeck, Georges L. Mvumbi, Marie-Pierre Hayette

Article Infectious Diseases

Economic costs analysis of uncomplicated malaria case management in the Peruvian Amazon

Diamantina Moreno-Gutierrez, Angel Rosas-Aguirre, Alejandro Llanos-Cuentas, Joke Bilcke, Jose Luis Barboza, Marie-Pierre Hayette, Juan Contreras-Mancilla, Kristhian Aguirre, Dionicia Gamboa, Hugo Rodriguez, Niko Speybroeck, Philippe Beutels

Article Microbiology

A Recurrent Mutation at Position 26340 of SARS-CoV-2 Is Associated with Failure of the E Gene Quantitative Reverse Transcription-PCR Utilized in a Commercial Dual-Target Diagnostic Assay

Maria Artesi, Sebastien Bontems, Paul Gobbels, Marc Franckh, Piet Maes, Raphael Boreux, Cecile Meex, Pierrette Melin, Marie-Pierre Hayette, Vincent Bours, Keith Durkin

Article Dermatology

Evaluation of the new Id-Fungi plates from Conidia for MALDI-TOF MS identification of filamentous fungi and comparison with conventional methods as identification tool for dermatophytes from nails, hair and skin samples

Rosalie Sacheli, Anne-Sophie Henri, Laurence Seidel, Marie Ernst, Rajae Darfouf, Caroline Adjetey, Marjorie Schyns, Ludwig Marechal, Cecile Meex, Jorge Arrese, Marie-Pierre Hayette

Article Multidisciplinary Sciences

Capillary whole-blood IgG-IgM COVID-19 self-test as a serological screening tool for SARS-CoV-2 infection adapted to the general public

Serge Tonen-Wolyec, Raphael Dupont, Salomon Batina-Agasa, Marie-Pierre Hayette, Laurent Belec

Article Multidisciplinary Sciences

The lack of K13-propeller mutations associated with artemisinin resistance in Plasmodium falciparum in Democratic Republic of Congo (DRC)

Doudou Malekita Yobi, Nadine Kalenda Kayiba, Dieudonne Makaba Mvumbi, Raphael Boreux, Sebastien Bontems, Pius Zakayi Kabututu, Patrick De Mol, Niko Speybroeck, Georges Lelo Mvumbi, Marie-Pierre Hayette

Article Microbiology

Belgian National Survey on Tinea Capitis: Epidemiological Considerations and Highlight of Terbinafine-Resistant T. mentagrophytes with a Mutation on SQLE Gene

Rosalie Sacheli, Saadia Harag, Florence Dehavay, Severine Evrard, Danielle Rousseaux, Akole Adjetey, Laurence Seidel, Kim Laffineur, Katrien Lagrou, Marie-Pierre Hayette

Article Medicine, General & Internal

Evaluation of Two Rapid Antigenic Tests for the Detection of SARS-CoV-2 in Nasopharyngeal Swabs

Ysaline Seynaeve, Justine Heylen, Corentin Fontaine, Francois Maclot, Cecile Meex, Anh Nguyet Diep, Anne-Francoise Donneau, Marie-Pierre Hayette, Julie Descy

Summary: The study evaluated the performance of two new antigen detection kits in laboratory settings, with HeRDT showing a higher sensitivity compared to CoRDT. Despite excellent specificity, the poor sensitivity of CoRDT makes it unsuitable for rapid diagnosis of COVID-19.

Article Microbiology

Pharmaco*kinetic Variability and Target Attainment of Fluconazole in Critically Ill Patients

Ruth Van Daele, Joost Wauters, Katrien Lagrou, Raphael Denooz, Marie-Pierre Hayette, Matthias Gijsen, Roger J. Brueggemann, Yves Debaveye, Isabel Spriet

Summary: The study aims to define variability and target attainment for fluconazole exposure in critically ill patients. The results show that a considerable number of measured fluconazole concentrations were below the predefined target trough concentrations, mainly influenced by factors such as the administered dose, augmented renal clearance, and renal replacement therapy.

Article Microbiology

In-Depth Longitudinal Comparison of Clinical Specimens to Detect SARS-CoV-2

Justine Defeche, Samira Azarzar, Alyssia Mesdagh, Patricia Dellot, Amandine Tytgat, Fabrice Bureau, Laurent Gillet, Yasmine Belhadj, Sebastien Bontems, Marie-Pierre Hayette, Raphael Schils, Souad Rahmouni, Marie Ernst, Michel Moutschen, Gilles Darcis

Summary: Testing and isolation of COVID-19 patients are crucial in controlling the pandemic, with PCR tests being the gold standard for diagnosis. The study compared the sensitivity of different sample specimens and found that nasopharyngeal swabs were most sensitive, while throat washing showed better performance at later time points.

Article Virology

Evaluation of Screening Program and Phylogenetic Analysis of SARS-CoV-2 Infections among Hospital Healthcare Workers in Liege, Belgium

Majdouline El Moussaoui, Nathalie Maes, Samuel L. Hong, Nicolas Lambert, Stephanie Gofflot, Patricia Dellot, Yasmine Belhadj, Pascale Huynen, Marie-Pierre Hayette, Cecile Meex, Sebastien Bontems, Justine Defeche, Lode Godderis, Geert Molenberghs, Christelle Meuris, Maria Artesi, Keith Durkin, Souad Rahmouni, Celine Gregoire, Yves Beguin, Michel Moutschen, Simon Dellicour, Gilles Darcis

Summary: Healthcare workers, especially nurses, paramedics, and laboratory staff handling respiratory samples, are at higher risk of SARS-CoV-2 infections. Phylogenetic analysis reveals that most positive samples were independently introduced into the hospital.

Article Virology

Cluster Analysis Identifies Distinct Patterns of T-Cell and Humoral Immune Responses Evolution Following a Third Dose of SARS-CoV-2 Vaccine in People Living with HIV

Majdouline El Moussaoui, Salome Desmecht, Nicolas Lambert, Nathalie Maes, Joachim Braghini, Nicole Marechal, Celine Quintana, Karine Briquet, Stephanie Gofflot, Francoise Toussaint, Marie-Pierre Hayette, Pieter Vermeersch, Laurence Lutteri, Celine Gregoire, Yves Beguin, Souad Rahmouni, Michel Moutschen, Daniel Desmecht, Gilles Darcis

Summary: By analyzing immune parameters, we found heterogeneity in immune responses to the COVID-19 vaccine among people living with HIV. Different patterns of immune response may require distinct strategies for intervention. Prior or breakthrough infection enhances the vaccine efficacy and should be considered in global vaccine strategies for people living with HIV.

Article Microbiology

Hospital Laboratory Survey for Identification of Candida auris in Belgium

Klaas Dewaele, Katrien Lagrou, Johan Frans, Marie-Pierre Hayette, Kris Vernelen